Using the microscope to grade compost and compost extract

This is using Elaine Ingham’s microscopy techniques to investigate thermal compost – some of what I saw. I am at an early stage of being able to do this, so any errors are mine and not Elaine Ingham’s 😉 The principle is to classify organisms by their morphology – aerobic fungi tend to have a colour, diameter wider than 2.5µm and/or have uniform septa. Spiral structures are bad, indiciative of anaerobic conditions, and ciliates (hairs all over the body) also indicate anaerobic – bad- conditions. Apart form the spirochete most of these are good.

This is on a 5x dilution, the recommended intial conditions (use 1ml of compost and make up to 5ml total with water left to stand so the chlorine has gone).

Weatherizing a Raspberry Pi Camera with a Microscope slide

Sticking a Raspberry Pi camera exposed to the elements doesn’t do it any good over time, resulting in the hazy crazed lens problem.

Flare on the camera lens after a year in the open
Flare on the camera lens after a year in the open

The solution is to put some glass in front of the lens – and indeed this is exactly what this commercial outdoor spec little lipstick CCTV camera does

it's hard to see, but there is a round glass against an O ring in gront of the camera lens on this weatherpoof camera, which has spent several years outside and still works well
it’s hard to see, but there is a round glass against an O ring in gront of the camera lens on this weatherproof camera, which has spent several years outside and still works well

I discovered this when I took it apart to unscrew the lens a bit to make a close focus. And then cracked the glass refitting it as the lens stuck out too much. If you ever need a flat round piece of glass, search for watch crystal on ebay and they are to be had in lots of diameters. A watch crystal is apparently a term for the glass on a watch as well as the 32,768 Hz timing quartz crystal. A flat watch crystal repaired this camera.

The direct exposure of the camera lens to the elements is the biggest weakness of the now-defunct PICE weatherproof Pi case. But it is easily rectified now, using a piece of flat glass fitted with Sugru or Milliput putty. I used sugru and a cut down microscope slide, since I didn’t want to buy another watch crystal when microscope slides are optically flat and cheaper. It is a lot easier to cut glass under water, and you can remove the viciously sharp edges using a cheap diamond sharpening stone to smooth the cut edge and chamfer the corner.

Microscope slide fitted with Sugru to shed the water and seal the camera from the elements
Microscope slide fitted with Sugru to shed the water and seal the camera from the elements

Continue reading “Weatherizing a Raspberry Pi Camera with a Microscope slide”

Making Compost Extract

The easiest and low-tech way of adding the microorganisms from compost is to extract them fro mthe compost using water and a mesh, then spray the water  – in our case using watering cans. You can spread the compost itself, and there’s much to be said for that, but it’s more stuff to wrangle and needs to happen before you plant, ideally. Since we are going to test areas on already growing plants, extract it is. Compost tea is a way of getting more microbes out there, but it is technically harder and we don’t have the gear. Extract it is, then. The rate seems to be about a good handful per 5 gallons, we used half an IBC, ie about 500 liters, which is 110 gallons. So we need about 20 times as much

Start with a wodge of compost in a net curtain
Start with a wodge of compost in a net curtain
and some reasonably clean borehole water (so no worries on chlorine or chloramine)
and 500 litres reasonably clean borehole water (so no worries on chlorine or chloramine)

We made a bag by putting the compost in the middle of the net curtain material and tying up the top with a releasable cable tie.

You can see the brown of the humic acid leaching out of the compost into the water
You can see the brown of the humic acid leaching out of the compost into the water
the end result is a dark brown chocolate colour
the end result is a dark brown chocolate colour

Continue reading “Making Compost Extract”

using the microscope with the finished compost

The successful compost is ready – it has now fallen to roughly ambient temperature.

1507_otl_AS_fourweeklyunfortunately the temperature logger failed when I was on holiday so I don’t know what the profile was as it cooled down. And yes, it didn’t spend three times three days above 55C – more like three days and two days. There’s still more to learn here.

Time to look at this and see what sort of microbial stuff is in it. I shook this up with about 20 times the amount of water and put a drop on a slide

fungal hypah and bacteria
fungal hypha and bacteria

According to Elaine Ingham’s rules of thumb this is probably a good sort of soil fungus, because if the little round cocci are 1µm in diameter the fungal hypha is about 4µm. I could see that this one was slightly tan coloured, but the incandescent lamp of the microscope plays havoc with the white balance of the camera, making everything bright yellow.

1507_hypha_DSCN2669_lznThis next one is narrow and clear, so not good in the morphology  rule of thumb that fungi < 3µm in diameter and clear are undesirable soil fungi.

I saw no protozoa or micro-arthropods. That’s either because there aren’t any or because I didn’t recognise them. The dilution is high, – it appears that Ingham starts at 5:1 so I’m four times less likely to see these at 20:1.

 

concentrated compost activators are hard to use

The high-nitrogen activator should typically be about 10% of the composting materials. These are typically animal wastes – I have used real chicken crap, pelletised chicken crap, and clover. With the chicken manure each time I have scored a fail, whereas the clover was a success.

pelleted chicken manure
pelleted chicken manure

I suspect the trouble is that it’s hard to mix a concentrated activator properly. For starters it’s not pleasant to do, which discourages it being turned in right. The pelletised stuff is easy to distribute evenly, but even then it seems to lead to localised action.

pellets seem to turn white
pellets seem to turn white

The pellets seem to go white, like dog crap used to go white when left on the footpath in the 1970s. This leads to a fast and furious burn on the composting front, but with no staying power

pelletised chicken crap - leading to a fast ramp up but no staying power
pelletised chicken crap – leading to a fast ramp up but no staying power – the dip was when it was turned

The clover was more evenly spread – somehow I need to find a way of spreading the others more evenly. Or maybe go for the urine, preferably from carnivorous humans (there is more N in protein). In Ben Easey’s Practical Organic Gardening (Faber, 1955) he says dilute this with water 1:20 which should make for a better distribution. So I’m going to steer clear of using crap, because I am a wuss and don’t like dealing with it and it’s too concentrated anyway. Clover or urine will be my activators of choice 😉

Joanne’s note Oct 2016: We subsequently (in later heaps) used pelleted chicken manure mixed with water and stirred into a slurry. It took a lot of water to do this! Poor old Richard has a very sensitive sense of small (tough on a small farm with animals!) so he had to leave the rest of the team to finish up building the heap when we started to add the slurry…

Composting win

If at first you don’t succeed, try again 🙂 The requirements of Elaine Ingham’s thermal composting are quite demanding, keeping the heap at over 55C for more than three days to kill weed seeds and pathogens. The previous attempt got really close then seemed to dry out, this appears to be a issue with using a lot of woodchip which is a difficult material to wet. This time I used less of it.

more green material with this heap
more green material with this heap

I used a higher proportion of green material, and more of the high nitrogen clover too. I filled the wheelbarrows with woodchip and then added water until it overflowed, then left it to soak overnight

this achieved over 55C for three days - the dip is when this was turned
this achieved over 55C for three days – the dip is when this was turned on Thursday evening

After it had been over 55C for three days I turned this heap, wetting the material as it was turned over. I should be able to turn this again on Monday, assuming it holds above 55C

Composting – take two

Time to put some of the learning from the last time into practice, with thanks to Polly for help with wrangling the materials –

Raw materials
Raw materials

The clover which it the high-nitrogen component because it fixes N from the air is on the black plastic. Loads of wood chip is in the bin and the wheelbarrow. First we put sticks in the bottom to improve airflow because the whole point of thermal compost is to keep things aerobic

base of sticks to improve airflow
base of sticks to improve airflow

We needed to wet the material. In the video tutorials Ingham recommends standing the material in water overnight. We wetted is using a fine spray on the hose

Lucia and Polly soaking the woodchip
Lucia and Polly soaking the woodchip

A heck of a lot of material goes into a compost pile – thanks to Polly and Lucia who helped at the working party Continue reading “Composting – take two”

Nitrogen Fixing Clover Nodules

We have a lot of clover, which as Cotswold Seeds describe, are plants that fix nitrogen from the air in combination with Rhizobium bacteria living in root nodules. Since I’m going to use this for the high Nitrogen component instead of chicken crap I wanted to see if there were any active root nodules – after all if there were no bacteria or we had used nitrogen fertiliser there would be no nodules.

Clover root nodules
Clover root nodules

The good news is there are root nodules – so the clover is ready to fix nitrogen. The first  sample nodules have no red in them, even when dissected, which seems to mean they weren’t actually fixing nitrogen. This only starts when the soil temperature is above 8C. However, taking a second sample from the same patch showed better results.

opened nitrogen fixing root nodule
opened nitrogen fixing root nodule shows a distinct red colour

Looks like with these you have to open the nodules to see the red colour of leghemoglobin , they’re not particularly pink from the outside. Leghemoglobin transports the nitrogen to the plant from the nitrogen fixing bacteria.

Composting fail – what went wrong

Getting ready to have another go at composting the Elaine Ingham way after the first couple of attempts at thermal compost didn’t go right, I dissected the old material to find out why.

the heap dropped to about a quarter the original size
the heap dropped to about a quarter the original size

On the plus side, the material reduced in volume to 25% of the original volume. That in itself might be telling me something, in that perhaps i hadn’t packed down the original pile enough – the composting bugs are small, and they need to be in contact with the compost to munch it, there is such a thing as too much air gap 😉

looks particularly dry, except in the very middle
looks particularly dry, except in the very middle

Only the very middle was wet, and did not smell, so it looks like this pile largely dried out from the outside, it probably didn’t go anaerobic, and indeed was active enough to nut the smell of the chicken crap used as the high N part.

Continue reading “Composting fail – what went wrong”

Composting – the Elaine Ingham way

Compost isn’t something I’d even given much thought to, I got it in bags from B&Q and job done.

Compost
Compost. On a farm scale you get it wholesale, not from B&Q 🙂

A long time ago I never bothered and used garden soil, perhaps an instinctive predilection towards natural farming – as exemplified in shumei.

I visited the guys doing Shumei farming in Wiltshire - being Japanese they had this image of Mount Fuji on the site, though the December rain doesn't do it justice
I visited the guys doing Shumei farming in Wiltshire – being Japanese they had this image of Mount Fuji on the site, though the December rain doesn’t do it justice

At school I learned that soil has micro-orgnaisms that somehow worked symbiotically with the plants, but pretty much everything in the decades that passed seemed to run counter to that – perhaps my schoolbooks were from an earlier era.

Continue reading “Composting – the Elaine Ingham way”